VariLink™ Technology - The new standard for detecting genetic variation
VariLink™ technology enables the most comprehensive view of genetic variation on a short-read NGS platform with a simple, streamlined workflow. Because phase information is preserved, VariLink™ technology delivers an unprecedented view of genetic variation that typically requires multiple assay types to achieve. The capture of long-range information in parallel with phase informative primary sequence enables the detection of large structural variants, SNPs, indels and CNAs in a simple NGS library preparation workflow. Genetic markers commonly detected using multiple assay formats can now be detected from a single NGS sequencing run saving time and effort.
VariLink™ Technology - The new standard for detecting genetic variation
VariLink™ technology enables the most comprehensive view of genetic variation on a short-read NGS platform with a simple, streamlined workflow. Because phase information is preserved, VariLink™ technology delivers an unprecedented view of genetic variation that typically requires multiple assay types to achieve. The capture of long-range information in parallel with phase informative primary sequence enables the detection of large structural variants, SNPs, indels and CNAs in a simple NGS library preparation workflow. Genetic markers commonly detected using multiple assay formats can now be detected from a single NGS sequencing run saving time and effort.
Ideal for:
- Oncology research programs: Create comprehensive panels capturing long structural variants, SNPs, indels and CNAs in a single NGS-based assay.
- HLA Typing: Assess true haplo-identity through long-range phasing across the entire 4Mb HLA region.
- Haplotype calling: Determining haplotype for genes, such as CYP2D6, where accurate calling is commonly confounded by neighboring pseudogenes. Phase informative reads enable haplotype ambiguities to be minimized.
Capture more information with every sequencing run.
Short-read next-generation sequencing (NGS) platforms are blind to genetic variants such as large SVs due to read-length limitations. VariLink NGS libraries capture long-range information through a next generation proximity ligation approach greatly expanding the types of genetic variants detectable. In addition, the datatype preserves phase information alongside the primary sequence enabling haplotype determination.
Simple workflow completed in a single day.
Fully compatible with existing short-read NGS platforms, VariLink libraries can be completed in a single working day. Starting with fresh frozen cells, sequencing ready libraries are ready in less than 6hrs.
Data compatible with common bioinformatic tools and approaches
Paired-end sequencing of VariLink libraries generates data that mimics the uniform coverage of standard shotgun libraries. Consequently, many standard data analysis tools (e.g. GATK) and approaches are compatible with the datatype. Cantata Bio customer support scientists are available to provide guidance on specific data analysis needs you may have.
Features and Benefits
|
Feature |
Benefit |
|
Broad capture of large structural variants, SNPs, indels and CNAs |
Most comprehensive view of genetic variation in a single assay |
|
Can be deployed genome-wide or with targeted panels |
Create an assay specifically tailored to your content needs |
|
Enables capture of long-range SNP phase information alongside primary sequence |
Minimize haplotype ambiguities. Fully phase across highly polymorphic regions such as HLA |
|
Process samples in a single working day |
Complete sample preparation within a typical laboratory work shift |