Take your ChIP Seq data to the next level

HiChIP MNase Kit

Flowchart illustrating DNA analysis process: Crosslink, Fragment, ChiIP, Ligate, Reverse Crosslinks, Sequence, with associated diagrams of molecular steps. Descriptions include cell input, no sonication, antibody validation, and preparation methods.

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See Your ChIP-seq Data in a Whole New Dimension

The Dovetail HiChIP MNase Kit combines the benefits of ChIP-seq with Hi-C, a proximity ligation method that captures long-range interactions using standard Illumina paired-end sequencing. Query protein-directed chromatin conformation mediated by specific proteins of interest and see your ChIP-seq data in a whole new dimension.

Genomic data visualization displaying chromosome 7 with high-throughput sequencing reads, ChIP-seq and HiChIP interaction profiles, and gene annotations for CREB5, TRIL, CPVL, CHN2, CHN2, LOC648762, WIP3, SCRN1, and PLEKHB.

With a ChIP-seq kit, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.


HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.

Nucleosome-Level Resolution

Graph showing peak distortion through regulatory elements with three tracks: CTCF-ChIP-seq in green, CTCF-DTG HiChIP in blue, and CTCF mbol HiChIP mbol RE sites in red. Each set displays data across chromosome 20 regions with specific peaks.
Graph showing loss of peak signal due to different types of restriction enzymes in DNA sequencing, with lines labeled CTCF-ChIP-seq, CTCF-DTG HiChIP, and CTCF mbol HiChIP mbol RE sites.

Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).

Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.

Two heatmaps labeled A and B showing distance from CTCF site in kilobase pairs on axes, with a dense cluster near the center, representing genomic data.

Validated Antibodies

Table comparing validated antibodies, with columns for antibody name, supplier, and part number, divided into two sections.

Kit Details

HiChIP MNase Kit | Full Solution​ HiChIP MNase Kit | Full Solution​
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HiChIP MNase Kit | Full Solution​

Dr. Orlando Contreras-López – Research Scientist at NGI Stockholm – SciLifeLab

"The quality of service from the Dovetail® scientific support team is one of the best we have experienced from vendors. They show a great deal of technical knowledge and a genuine care in supporting our efforts to prepare high quality proximity libraries using Dovetail® kits."

Webinar Highlight

Our Head of Customer Success, Dr. Myriam Elkhawand, will discuss how Dovetail® HiChIP data enables gene regulation to be understood at a deeper level. During this webinar you'll learn:

  • The role of genome conformation in gene regulation

  • Why topological interactions paint a more complete picture of gene regulation

  • How topology can be used in development, gene regulation, and disease studies

  • How to combine the 3-Dimensional data of Hi-C with ChIP-seq

Watch Overview Here