Dovetail® Micro-C Kit
900+ Libraries Created
Hi-C is a powerful tool for understanding 3D chromosome architecture, how chromatin topology influences cellular function and how topological changes contribute to disease. However, traditional Hi-C, relying on sequence biased restriction enzymes, has significant limitations. The Dovetail® Micro-C Assay addresses many of these limitations.
The MNase Advantage
The Dovetail Micro-C Kit utilizes micrococcal nuclease (MNase), generating highly uniform fragments and uniquely capturing nucleosome positioning and an even coverage across the genome. The result? Increased mapping resolution aligned with nucleosomes. Micro-C detects higher-order features, such as chromatin loops, using proximity ligation data by enriching long-range informative reads and capturing chromatin interaction frequency.
With only requires 800 M reads, Dovetail® Micro-C Kit generates 1 kb matrices. Compared to the 1.2-1.6 billion reads required by restriction enzyme-based Hi-C methods, we greatly decrease sequencing costs.
3D genome architecture at the nucleosome level
Depicted above, the Micro-C MNase based assay captures nucleosome-level resolution, proximity-ligation information.
The Micro-C Difference
Breaking the Resolution Barrier: New Workflows Defining Chromatin Architecture at the Nucleosome Level
The creators of the Dovetail™ HiChIP and Micro-C assays share their experiences and impetus for developing these game changing assay kits for epigenetic research. Details on the how the kits work, their benefits and the ways they see these new technologies impacting genomic research will be shared.
Explore the data
The contact matrices to the right show an enrichment of contacts compared to traditional Hi-C approaches due to Micro-C’s greater signal-to-noise. All matrices were generated from 800 million total read pairs and contacts were balanced.
Micro-C demonstrates increased sensitivity for topological features at lower sequencing depths. The difference is visible to the naked eye.
Micro-C produces more uniform coverage of the genome
The genome coverage map to the left compares different enzyme approaches and sequencing coverage. The Micro-C assay overcomes sequence gaps present in traditional Hi-C data because Micro-C is not limited by low restriction site density.
The MNase-based approach of the Micro-C assay captures accurate and non-biased chromatin topology across the entirety of the genome.
Kit Overview
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Resolving the 3D landscape of transcription-linked mammalian chromatin folding
Distinct Classes of Chromatin Loops Revealed by Deletion of an RNA-Binding Region in CTCF
Nature: Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome
Condensin-Dependent Chromatin Compaction Represses Transcription Globally during Quiescence
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8 Reactions
Validated Samples
Mammalian cells
Labeling
Research Use Only
Module 1 of 2
Storage
2°C to 8°C
Content
TE Buffer, pH 8.0
10x Wash Buffer
TWB Solution
2x NTB Solution
LWB Solution
NWB Solution
Chromatin Capture Beads
Crosslink Reversal Buffer
Streptavidin Beads
10x RBC Lysis Buffer
20% SDS
Module 2 of 2
Storage
-30°C to -10°C
Content
MNase Enzyme Mix
10x Nuclease Digest Buffer
100 mM MgCl2
0.5 M EGTA
End Polishing Enzyme Buffer
End Polishing Enzyme Mix
Intra-Aggregate Ligation Buffer
Intra-Aggregate Ligation Enzyme Mix
Bridge5x Bridge Ligation Buffer
5x Bridge Ligation (T4 DNA Ligase)
Proteinase K
250 mM DTT
HotStart PCR Ready Mix
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Services
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For those looking for a comprehensive genome-wide view to 3D chromatin architecture, Micro-C services enable detection and visualization of topological features including A/B compartments, TADs, and chromatin loops with the Dovetail® Micro-C Assay.
Reach out to the team to start your project today. -
The Dovetail® Pan Promoter Panel Services are ideal for researchers ready to focus their 3D genomics data on specific regions, such as promoters.
This service identifies promoter mediated looping events at a fraction of the sequencing cost of other whole genome approaches. The targeted approach enables researchers wanting robust replication, increased statistical power, and expanded sample sizes.
Learn more about the Dovetail® Pan Promoter Panel Here
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Add annotation for deeper insights