Dovetail® Omni-C Kit

1000+ Genome Assemblies

Whether you are interested in species conservation, studying the intricacies of evolution or looking to improve the efficiencies of agricultural biology, the first step. Dovetail Genomics continues to be the leading provider of high quality genome assemblies.

With over 900 publications, you can trust our proven kits and services to produce the highest quality, phase informed assembly possible all enabled by Dovetail’s Linked-read technology.

Produce the highest quality genome assembly.

The ultimate scaffolding tool

Dovetail® Omni-C® data captures long range interactions while delivering uniform sequence coverage—enabling haplotype phasing

The Omni-C kit moves away from the limitations and bias faced by traditional restriction-enzyme based Hi-C, leveraging the long-range interactions to get a complete picture of the genome.

Omni-C data provides the most complete genome-wide view for all genome assembly, phasing, structural variant, and SNP research questions.

Line graphs showing genomic coverage data for different sequencing methods: Omni-C, Hi-C Multi-RE, and Hi-C 1-RE across three columns titled 'Genomic Coverage', 'Coverage at GATC', and 'Coverage at GANTC'. Each row compares the three methods with distinct line styles and colors.

A New Standard: Chromosome scale haplotype aware assemblies made with HiFi and Omni-C technologies

Watch Overview

A haplotype-resolved assembly provides a biologically relevant view of your genome. Long-range sequencing approaches, such as PacBio’s HiFi technology, combined with Dovetail's Omni-C libraries make full chromosome scale haplotype-resolved assemblies possible.

Workflow diagram titled 'Dovetail's Diploid Assembly Workflow' illustrating steps for diploid genome assembly, using numbered circles and color-coded process boxes, including sample receipt, library links-read, assembly, scaffolding, completeness evaluation, and sequencing/annotation.

Polyploid genomes are among the most difficult to assemble, yet many species of critical importance to evolutionary biology, conservation, and agriculture fall into this category. Because polyploids contain multiple homologous chromosomes, it is often challenging to distinguish between copies. Each gene may be represented by multiple alleles with subtle sequence differences, and resolving these variants with accuracy is essential to linking genotype to phenotype. Achieving this requires additional phasing information during the assembly process.

Dovetail® Genomics overcomes these barriers by combining information-rich Linked-Read data—capturing primary sequence, long-range contacts, and SNP phase information in a single sequencing run—with our state-of-the-art assembly pipeline. This unique approach has enabled us to successfully assemble high-quality genomes for a wide range of polyploid species, delivering the resolution researchers need to advance discovery in complex systems.

Polyploid Assembly

Powering true diploid assemblies

Despite decades of sequencing advancements, many human, plant, and animal reference genomes remain haploid because they lack phase information.

By combining shotgun sequence coverage with the ability to accurately phase SNPs genome-wide, Omni-C powers true diploid (haplotype-resolved) assemblies.

This enriched dataset delivers a more complete view of genetic complexity, enabling investigation of:

  • Species hybridization

  • Cis vs. trans mutations

  • Allele-specific expression

  • Structural variation

  • Linkage disequilibrium patterns

  • Segmental duplications

  • Complex gene families

Flexible options: kits or services

Run Omni-C in your lab, or leverage our comprehensive services team, who bring experience across hundreds of diverse taxa.

Kit Details

Omni-C® Kit | Full Solution Omni-C® Kit | Full Solution
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Omni-C® Kit | Full Solution

Services

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Start Your Project Today

  • The Dovetail® LinkPrep™ Kit powers true diploid assembly through shot-gun sequence coverage and the ability to accurately phase SNPs genome wide enabling the production of fully scaffolded haplotypes. Take advantage of our comprehensive services offerings and the expertise our services team has developed from working with hundreds of diverse taxa.

    Reach out to start your project today!

  • Polyploids are the most challenging genomes to assemble, yet, many species of great importance to the evolution, conservation and agricultural biology communties fall into this category. Since polyploid genomes contain multiple homologous chromosomes, this makes it challening to distinguish between the different copies. Polyploid organisms can carry multiple alleles for each gene, each differing in sequence. Resolving these allelic variants accurately is critical to understanding phenotype and requires additional phasing information during the assembly process.

    Due to these challenges, polyploid genome assemblies have been out of reach for all but the most experienced labs. Even then, the results commonly are unable to capture the full complexity.

    Leveraging the information rich Dovetail® LinkPrep™ data, a datatype that captures primary sequence, long-range contacts and SNP phase information in a single sequencing run, and a state-of-the-art assembly pipeline, Dovetail® Genomics is successfully assembling genomes for a wide assortment of polyploid species.

  • Add annotation for deeper insights

    A genome assembly is only as valuable as the biological insights it unlocks. Genome annotation, however, is complex, laborious, and time-consuming—especially without a closely related reference genome. With Dovetail® Genome Annotation Services, you can streamline this step and accelerate your understanding of the genetic blueprint underlying your study.

    Explore our genome annotation partnership offering with Goenomics GMBH.

  • "Our centre provides Genomic services to the scientific community over a wide range of research interests. The customer success team at Dovetail® goes above and beyond to help us through any questions or concerns we have in the course of using Dovetail® kits, whether it is for validated or non-validated sample types. This helps to ensure successful outcomes for our research community."

    Tonia Russell,
    Manager of Next Generation Sequencing - Long Read Services at Ramaciotti Centre for Genomics

  • "We are extremely happy with Dovetail’s Omni-C genome assembly of Japanese stiltgrass, now published in Genome Biology and Evolution. Invasive plant species are underrepresented among high-quality genomes, and we now have an invaluable resource for studying one of North America’s most damaging invasives. This has been a critical tool in our studies of trait variation, genomics, adaptation, and aspects of invasion biology that otherwise would have been impossible."

    Craig Barrett, West Virginia University

    https://academic.oup.com/gbe/article/13/11/evab238/6413638

  • Dovetail did an amazing job assembling the genome of annual water hyacinth (Eichhornia paniculata) with PacBio HiFi and Omni-C sequences. We got a chromosome level assembly with a very high BUSCO score (97.2%). We are using it for whole genome alignments to look for the structural variations that make up the tristyly supergenes.

    Haoran Xue, University of Toronto

  • "Based on a previously assembled genome sequence of a wild tomato relative Solanum habrochaites PI127826 using Dovetails Genomics technology helped us to obtain a chromosome-level assembly for most of our favorite wild tomato relative Solanum habrochaites Pi127826. Dovetails technology was nicely able to complement previous available 10X Genomics and Optical Mapping information yielding not only an improved assembly but also a genome annotation. Altogether, this new reference genome serves as a new foundation for our attempt to understand the basis of relevant traits into cultivated tomato."

    Marc Galland, University of Amsterdam

  • "Through the test of our several genome projects, Dovetail’s Hi-C kit has stable and excellent performance in plants, mammals and poultry. By using Dovetail Hi-C and HiRise assembly pipeline, we can obtain high-quality genomes at chromosome level. Taking eggplant as an example, we have obtained the highest quality eggplant genome so far, with contig N50=5.3 Mb and scaffold N50=93.9 Mb. In the future, we will use Dovetail’s Hi-C technology in more genome projects."

    Yongchao Niu, Biozeron Shenzhen, Inc.

  • "We have a project to sequence the genome of three Dugesia flatwom species, animals with around 2 Gbp genomes, for developing diverse studies on their evolution. The only genomes published till now for this group belong to a species from a close genus Schmidtea mediterranea for which, until very recently, only a highly fragmented genome was available. For the first two species of our project, Dovetail using PacBio and HiRise have generated a chromosome level assembly, which seems to me nearly unbelievable. I thank Dovetail for their professionality and good results. With these genomes we will be able to perform studies on chromosomal reorganizations and the effects of long-term fissiparity."

    Marta Riutort, Biodiversity Research Institute (IRBio)

  • "Highly heterozygous bivalve genomes are notoriously hard to assemble, even using long-read technologies. Dovetail’s Omni-C technology allowed us to assemble a high-quality chromosome-level genome forMytilus edulisdirectly from a draft long-read assembly. This high-quality genome will enable us to study presence-absence variation in the appears to be a key element of the adaptability of the genusMytilusand generate important genetic resources for these commercially important bivalves."

    Tiago Hori, Atlantic Aquafarms

  • "We sent Dovetail tissue samples and they sent us a high quality genome, with prompt and expert technical support, at a great price. Can’t ask for anything else and we’re planning to use their services again. At the Harris lab (www.fishbonelab.org) this genome will enable comparative genomics and population genetics analyses to unravel the ocean quahog’s secrets of exceptional longevity, up to 507 years."

    Stephen Treaster, Harvard Medical School

  • "We generated a reference-quality genome Solemya velum (Bivalvia: Protobranchia) to allow us to test hypotheses about mollusc genome evolution and the symbiotic interactions in marine molluscs. 10X Genomics, PacBio Long-reads and Hi-C data was leveraged for molluscan genome assembly. Iterations and combinations of each data type have resulted in a 2.4 GB reference genome with a scaffold N50 of 200 MB and a BUSCO completeness score of 93.7%."

    Vanessa Gonzalez, Smithsonian Institution