Galileo ONE

A Sample-to-Result Integrated Workflow

Galileo ONE is a metagenomic NGS (mNGS) platform that takes the user from a primary sample to quantitative results for over 1300 microbes. This turnkey workflow combines the Galileo ONE Sample Preparation Kit with automated analysis and reporting using our cloud-based Galileo Analytics and assumes the user has access to an Illumina sequencer.

Cutting-Edge Technology in Your Own Lab

Galileo ONE empowers users to easily bring mNGS technology in-house, providing greater flexibility and control over assay parameters as well as shorter turnaround time. No more black box pipelines or waiting for samples to ship. And because Galileo ONE is a fully integrated workflow with full process controls included, it eliminates the need for bioinformatics expertise to get started.

Get the Most Insight Out of Every Sample

Unlike a targeted approach which provides limited insight, Galileo ONE is a truly unbiased approach to microbial detection. The platform can detect organisms that cannot be cultured, as well as those that require special conditions to grow. And with parallel DNA and RNA workflows, Galileo ONE enables detection and quantification of greater than 99% of known human pathogens* from a single sample using one test.

*Foresight. Infectious Diseases: Preparing for the Future. Office of Science and Innovation; London, UK : 2006.

Detect All in One Sample

Bacteria
Viruses
Fungi
Parasites

All Nucleic Acid Types

Double-stranded DNA
Single-stranded DNA
RNA

Integrated Controls

Background control
Internal control
External positive control

Get Started Today

Access to a sequencer is all that is required to begin using the Galileo ONE workflow for mNGS analysis.

Parameter

Galileo ONE Features

Galileo ONE Benefits

Sample Type

Plasma, CSF, other liquid sample types

Minimal optimization to analyze multiple liquid
sample types.

Nucleic Acid Type

DNA (double- and single-stranded) and RNA

Detects all microbial types, including RNA viruses.

Minimum Input Volumes

1.2 mL (0.8 mL for DNA, 0.4 mL for RNA)

Maximizes analytical sensitivity while minimizing use of precious samples.

Sequencing

Compatible with all Illumina platforms

Utilizes sequencers found in most sequencing cores and labs.

Analytics

Cloud-based automated informatics pipeline

Ensures that only reference genomes meeting quality control standards are included in analysis.

Database

Proprietary and manually curated set of
reference genomes.

Integrated control scheme enables real-time quality control monitoring of sequencing runs
and libraries.

Control Scheme

Full-process internal and external run controls, plus quantification controls.

Reporting

Microbial identification and quality control reports generated for each library.

The Galileo ONE Sample Preparation Kits are available in three configurations: DNA-only, RNA-only, or Combo DNA+RNA. Each kit includes reagents and consumables needed to generate sample libraries, plus external, internal, and background controls. The Training Kit supports live and web-based training by our in-house experts on the Galileo ONE workflow and enables seamless implementation and routine use in your facility. Access to Galileo ONE Analytics is included for each kit through our user-friendly web app and customizable APIs.