Dovetail® VariLink™

High Sensitivity Structural Variant Detection

Structural Variation Presents a Unique Detection Problem For Many Technologies

No single technology can fully capture the breadth of genetic variation. Structural variation, in particular, is incredibly diverse and complex, encompassing a vast range of sizes and configurations. This challenges researchers, as they must rely on multiple technologies to detect and validate these variants while also using WGS for smaller SNVs and INDELs. The Dovetail® VarilinkTM Assay overcomes these limitations.

Capture SVs With High Sensitivity Without Sacrificing SNVs and INDELs

Proximity Ligation Enables High Read Support Through Breakpoint ‘Flanking’

The Varilink data structure leverages in situ DNA-DNA interactions within a cell's nucleus to generate ultra-high supporting evidence. By accumulating breakpoint evidence through these interactions, Varilink achieves a non-linear increase in breakpoint signal with sequencing coverage. This leads to enhanced sensitivity, reduced false positives, and lower sequencing needs. In contrast, WGS approaches rely on reads spanning breakpoints, resulting in higher sequencing demands and a higher false positive rate.

VariLink Data Offers 100X Improved Sensitivity Over WGS

Proximity ligation methods, such as Varilink and Hi-C, excel at detecting structural variants (SVs) due to their enhanced breakpoint signal. Unlike WGS, which captures only 36% of the true SV set, these approaches maintain 100% detection rates even with reduced sequencing depth. Varilink’s optimized signal generation surpasses Hi-C, enabling complete SV detection at a remarkably low 0.1X genomic coverage.

The WGS-like Coverage of VariLink Data Captures SNVs and INDELs

The uniform coverage of VariLink data seamlessly captures SNVs and INDELs. Varilink read pairs can be directly analyzed using standard variant callers and delivers comparable results to WGS. Unlike other proximity ligation methods like Hi-C, Varilink's consistent coverage ensures accurate identification of these smaller variants.

Capture SVs Missed By Other Technologies, Without All the False Positives

Enhance Your Genetic VariationResearch with Flexible Solutions

Application

Genetic Variation

Large Structural
Variant Detection

Genotyping

Haplotype Phasing

Chromatin Topology

Enhancer Promoter Interactions

Whole Genome Ultra
High-Resolution Topology

Whole Genome Rapid
High-Resolution Topology

Protein Anchored Topology

Genetic Genome Assembly

Genome Scafolding

Hybrid Capture Compatible

Dovetail Assay

LinkPrep™

Micro-C

HiChIP

Pan Promoter

Omni-C

* Workflow also requires the Dovetail Micro-C Kit

Application

Genetic Variation

Large Structural Variant Detection Genotyping

Genotyping

Haplotype Phasing

Chromatin Topology

Targeted Enhancer-Promoter Interactions

Nucleosome-level Chromatin Topology

Whole Genome Rapid
High-Resolution Topology

Protein Anchored Topology

Genetic Genome Assembly

Genome Scaffolding

Hybrid Capture Compatible

Dovetail Assay

LinkPrep™

Micro-C

HiChIP

Pan Promoter

Omni-C

* Workflow also requires the Dovetail Micro-C Kit

	Application		Use		LinkPrep		Micro-C		HiChIP		Pan Promoter		Omni-C
Genetic Variation	Large Structural Variant Detection

* Workflow also requires the Dovetail Micro-C Kit