Dovetail Genomics helped me in my research aim to generatede novogenome assemblies for the wild relatives of an important fibre and oilseed crop, flax. High quality genomes are becoming an essential starting point for genomics research and Dovetail Genomics has fueled my entry into this field to learn more about the genomic changes associated with the domestication of flax. The process was simple, starting with sending a sample of fresh material, and Dovetail Genomics did the rest, providing a quick service turn-around and additional support as necessary. I am very pleased with the results.
Dovetail Hi-C produced incredible scaffold ordering for our assemblies of the diploid ancestral species of peanut,Arachis duranensisandA. ipaensis. The new orderings made even clearer the incredibly close relationships between the genomes of these diploid species and the tetraploid crop.
This is very impressive indeed! Those look like the 17 chromosomes we would expect. We will start looking at the assembly more in details and get back to you if we have any questions, but for now I would say this definitely exceeded expectations.
Dovetail Genomics assembled a kiwifruit genotype for us to chromosome level and this assembly exhibits impressive synteny to existing whole genome sequences of other kiwifruit genotypes. We are very happy with the results achieved using Dovetail Chicago and Hi-C libraries and their HiRise assembly.
I’m very happy I chose to work with Dovetail Genomics to sequence theZizaniagenome. They were friendly, knowledgeable, and very communicative when I had concerns, questions, or inquired about updates throughout the process. TheZizaniagenome sequence and assembly has already been vetted by several other professionals, who have commented on its high quality. This resource will continue to aid in the conservation of natural stands ofZizaniaas well as the dissection of the underlying genetic mechanisms of important agronomic traits associated with cultivatedZizaniavarieties.
I want to thank you and the rest of the Dovetail team for all of the work you have done for us in this project. I think we are all pleased with the quality of the data retrieved and your very professional attitude. It was a pleasure working with you. We might come back to you in the future in case we need more details for the publication of that genome assembly but I think your reports have covered most of what we need for that.
Dovetail successfully scaffolded the ancestral A and C genomes of oat from Sequel II contigs for our group (3+ Gb genomes). These are highly repetitive genomes, so I had my worries that they’d get to chromosome scale – but the resulting scaffolds looked great (7 complete chromosomes; telomere to telomere in many cases). Comparison to linkage maps confirmed the scaffolding with very few exceptions (which could have been a linkage mapping error). We more recently compared their Omni-C with their Hi-C methodologies – both produced chromosome level scaffolds of Bromus tectorum (cheatgrass) – another 3+ Gb genome). We are in the middle of comparing the two scaffolded genomes to see if we like one better than the other – but both look pretty good. Dovetail has always been easy to work with and we appreciate that they respond professionally to our concerns in a timely manner.
We are delighted by the results you provided to us! So thank to Dovetail, and thank you guys for managing the project so professionally! The paper presenting the chromosome-scale assembly ofP. verishas been published in Molecular Biology and Evolution (https://doi.org/10.1093/molbev/msac035)
Dovetail took what seemed like an impossible project — getting an assembly for a plant with a large, repetitive genome — and made it a reality. They collaborated with different bioinformatics groups & did additional analyses to make sure we got the best results possible.
I have been extremely happy with the service provided by Dovetail Genomics. The communication with them has always been very easy and, importantly, they are willing to help with difficult projects.
We are very much happy about the Ipomea biloba genome project with Dovetail. The final genome assembly is really good and the N50 value met our expectations.
Thanks to everyone at Dovetail Genomics for the buffalograss genome! It is amazing and so are all of you too!
The use of Dovetail Chicago, Hi-C and Hi-Rise enabled us to improve the N50 of the firstEragrostis curvulagenome assembly from 300 Kb up to 43 Mb. Having almost seven complete chromosomes out of the ten for the species, together with a high level of contiguity (since 83.7% of the genome size was contained in the first 14 scaffolds). This genome assembly have been used successfully to look for genes related to the reproductive mode, drought tolerance and forage quality.
The Dovetail approach was able to accommodate the high repeat content of the lettuce genome and identified chimeric scaffolds that were missed by genetic analysis. Our resulting assembly was greatly improved in accuracy and contiguity and serves as the reference genome for the community.
Our soybean research team (@AoE_GSPEI) is very satisfied with the Dovetail Hi-C library and HiRise analysis pipelines. It was very helpful in putting thousands of scaffolds into high-quality chromosomes. We are excited by the discovery of differences between wild and cultivated soybeans using the resulting high-quality, chromosome-scale wild soybean genome.
We sent Dovetail tissue samples and they sent us a high quality genome, with prompt and expert technical support, at a great price. Can’t ask for anything else and we’re planning to use their services again. At the Harris lab (www.fishbonelab.org) this genome will enable comparative genomics and population genetics analyses to unravel the ocean quahog’s secrets of exceptional longevity, up to 507 years.