Dovetail^® HiChIP MNase Kit

Genomic interactions matter. With a ChIP-seq kit, you only get chromatin information in linear space – meaning you may not be getting all the details you need to gain novel insights on how enhancer/promoter interactions and protein-directed chromatin architecture regulates gene expression.

HiChIP gives your ChIP-seq data a 3D boost so you can take your data to new dimensions. Use conformation contact information to explore how regulatory elements and the spatial relationships between regulatory elements can influence gene expression and drive disease progression.

Approximately 20% of the mappable human genome is blind to Hi-C when using restriction enzymes (RE), since signal can be unduly influenced based on the availability of restriction sites close to the protein binding site. The Dovetail HiChIP MNase Kit overcomes this by using micrococcal nuclease (MNase), an enzyme with sequence-independent endonuclease and exonuclease activities, to uniformly fragment chromatin and get the highest resolution 3D genome architecture mapping (150 – 200 bp conformation).

Don’t need ultra-resolution but looking for a way to save of sequencing costs? The Dovetail HiChIP MNase Kit generates data with higher signal-to-noise ratios so you can assess higher chromatin features, such as loop and chromatin interactions, at a fraction of the read depth.

Go from sample to a sequence in just 2.5 days. Dovetail Genomics kits are ready-to-use out of the box so you don’t need to spend time optimizing your protocol or qualifying reagents. A novel dual-crosslinking approach delivers unmatched capture of long-range interactions and library complexity so you can analyze chromatin interactions from nucleosomes to megabases. Plus, using MNase eliminates the need to sonicate your sample, removing one of the biggest sources of experimental variability. Dovetail is dedicated to your success and has a team of support scientist ready for consultation whenever you need it.

Dovetail Genomics has validated the following commercial antibodies so you can hit the ground running.