What is Targeted Enrichment?
Next-generation sequencing (NGS) is an extremely powerful tool for capturing genome-wide genetic information. However, more focused investigations can be conducted by hybridizing the sequencing library to capture probes that target pre-selected genomic regions. These captured duplexes are then "pulled down" prior to sequencing, thereby enriching your library. This results in a reduced amount of sequencing needed to achieve the desired coverage for your target regions.
Target Enrichment Applied to Proximity Ligation
Targeted enrichment can be combined with Linked-Read libraries. Historically, this has been done using Hi-C, but library bias caused by unevenly distributed restriction sites must be considered during enrichment panel design, as it can lead to coverage bias in the final library.
Dovetail® Linked-Read libraries eliminate this source of bias by using sequence-independent approaches, allowing standard panel design principles to be employed. While many commercially available targeted enrichment panels are compatible with our kits, only the Dovetail® Pan Promoter Enrichment Panels are specifically designed to study chromatin topology anchored at gene promoter sites.