Dovetail® Targeted Enrichment Panels

Study Chromatin Topology Anchored at Gene Promoter Sites.

Ideal For:
Targeted Genome Topology
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Targeted Enrichment Panels Video Overview


Enriching our understanding of gene regulation—a targeted view linking promoters with their regulatory elements.

The Dovetail® Pan Promoter Panels can be used when a targeted approach is desired because the hypothesis is more narrowly defined, reducing overall study costs. 

Often referred to as promoter capture Hi-C, this method enriches for genomic regions of interest by ‘pulling down’ pre-selected genomic regions with capture probes before next-generation sequencing (NGS).
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What is Targeted Enrichment?

Next-generation sequencing (NGS) is an extremely powerful tool for capturing genome-wide genetic information. However, more focused investigations can be conducted by hybridizing the sequencing library to capture probes that target pre-selected genomic regions. These captured duplexes are then "pulled down" prior to sequencing, thereby enriching your library. This results in a reduced amount of sequencing needed to achieve the desired coverage for your target regions.

Target Enrichment Applied to Proximity Ligation

Targeted enrichment can be combined with Linked-Read libraries. Historically, this has been done using Hi-C, but library bias caused by unevenly distributed restriction sites must be considered during enrichment panel design, as it can lead to coverage bias in the final library.

Dovetail® Linked-Read libraries eliminate this source of bias by using sequence-independent approaches, allowing standard panel design principles to be employed. While many commercially available targeted enrichment panels are compatible with our kits, only the Dovetail® Pan Promoter Enrichment Panels are specifically designed to study chromatin topology anchored at gene promoter sites.

View Gene Expression in a New Dimension

NGS data types such as ChIP-seq and ATAC-seq are blind to the 3D architectural structures that connect promoters and enhancers across long genomic distances. The power of promoter-targeted linked-read data is illustrated in the figure: interactions anchored at the MYC proto-oncogene promoter span more than 700 kb of sequence, identifying upstream regulatory regions. These regions align with enhancer (H3K27ac) and open chromatin (ATAC-seq) marks, while enhancer marks located closer to the MYC gene do not exhibit evidence of interaction.

Dovetail Pan Promoter Panel Loops, RNA-seq, H3k27ac, ATAC-seq

Sequence Less and Discover More, While Simplifying Data Analysis

Whole-genome experiments can require well over 1 billion read pairs to detect chromatin interactions. As a result, many studies are either underpowered or prohibitively expensive. By anchoring sequenced reads to promoters, sequencing depth requirements are reduced tenfold compared to non-targeted approaches, and the resulting data is more sensitive. 
Compared to whole-genome data, a greater number of interactions are detected, with virtually all data being informative of promoter regions. Data generated using the Pan Promoter Panel integrates easily with open-source tools, enabling simple and robust analysis.

sequence less, call more loops, highly targeted

Targeted genomic interactions provide novel insights across multiple applications

The Dovetail® Pan Promoter Panels can link non-coding variants and variants of uncertain significance (VUS) from GWAS data to specific gene content. This approach also expands understanding of oncogenic gene regulation through the discovery of enhancer hijacking events. Additionally, reduced sequencing requirements enable scalable 3D genomics experiments, allowing for larger sample sizes or more rigorous statistical analyses.

GWAS, 3D Genomics, Oncology

Easily Integrate Capture Hi-C Into Your Existing Workflow

Hybridization capture is performed on the final proximity ligation libraries immediately prior to sequencing, so you won’t need to make any change to the upfront proximity ligation workflow. Existing Dovetail Omni-C and Micro-C libraries can be target enriched without the need to create new libraries for this purpose.
Linked read assays

Capture Promoter-Driven Interactions with Comprehensive Panel Coverage

Pan Promoter Panels are optimized to capture all promoter regions across human or mouse genomes. This enables researchers to directly link data from ChIP-seq, ATAC-seq, or GWAS to their corresponding promoters—accelerating discovery and simplifying interpretation.
Panel content, genes, promoters, human, mouse

Customer Testimonials

“The Micro-C protocol is a breeze to follow. I really love the color-coded tubes, the "before you begin" sections, and the time estimates. Along with overall clear and concise writing, it is a fantastic user experience.”
Irene Duba, PhD. Candidate, Rockefeller University

Associated Dovetail® Kits

Dovetail® Micro-C Kit

Dovetail® Pan Promoter Enrichment Kit

Dovetail® Library Module for Illumina

Dovetail® Dual Index Primer Set #1 for Illumina

Specifications

Reactions
4 with up to 8 libs multiplexed/rxn
Validated Samples
Human or Mouse/span
Maximum # Libraries
32
Compatible Kits
Dovetail Omni-C & Micro-C Kits
Module 1 of 3 – content
Streptavidin Binding Beads
DNA Purification Beads
Binding Buffer
Wash Buffer 1
Wash Buffer 2
Module 1 of 3 – storage
2˚C to 8˚C
Labeling
For Research Use Only
Module 2 of 3 – storage
-25˚C to -15˚C
Module 2 of 3 – content
Universal Blockers
Blocker Solution
Hybridization Mix
Hybridization Enhancer
Amplification Primers
Capture Amplification Mix
Module 3 of 3 – storage
-25˚C to -15˚C
Module 3 of 3 – content
Pan Promoter Panel

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